Part:BBa_K4501014:Experience

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Applications of BBa_K4501014

We tested wether the transfection with this part provides exosomes with the ability to be purified by Ni-NTA affinity chromatography (based on the His-tag). For that, we transfected HEK293T cells and collect the supernatant after 24 hours with 0%FBS. Results show that a single shot of transient transfection (by electroporation) is enough to obtain low yield of purified exosomes.

Figure 1. Results on the purification by His-tag mediated affinity chromatogrpahy. (A) Levels of RFP expression on the transfected cells. (B) Yield of exosomes obtained by either ultracentrifuge or affinity chromatography. (C) NTA pattern of the size of particles purified on each condition.

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